Course Outline
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- Introduction
- Tissue Identification
- Connective Tissue
- Connective Tissue: Type I Collagen
- Connective Tissue: Type III Collagen
- Hepatocytes: Liver Epithelial Cells
- Kupffer Cells and Other Sinusoidal Cells
- True or False: Type III collagen, or reticular connective tissue, provides an architectural framework found in the form of a honeycomb network within ...
- Normal Histology
- Lobular Organization: Portal Tracts and Central Veins
- Portal Tracts: Bile Ducts
- Portal Tracts: Arterioles
- Portal Tracts: Portal Vein
- Parenchyma: The "Lobule"
- Parenchyma: Sinusoids
- True or False: The portal vein provides a large percentage of the blood supply to the liver; the remaining blood is supplied to the liver via the hepa...
- True or False: The epithelial cells of the liver are grouped together to form functional units referred to as liver lobules.
- Technical Preparation
- Fixative
- Rapid Tissue Processing: Transplant Patients
- Biopsy Cycle Tissue Processing
- Cassetting: Sponge versus Wrapping
- True or False: Liver biopsies are delicate and subject to grossing artifacts. Using sponges to secure biopsy tissue in a tissue cassette has been prov...
- Microtomy
- Artifacts
- Section Thickness
- Step Sections
- To aid in diagnosis, it is recommended that liver biopsy samples are sectioned at what micrometer (μm) thickness?
- Disease
- Stains
- Trichrome
- Reticulin
- Rhodanine
- Prussian Blue
- Oil Red O
- Immunohistochemistry and Immunoperoxidase
- True or False: Masson's trichrome stain is used to demonstrate connective tissue in liver biopsies, staining collagen blue.
- Reticular connective tissue cannot be visualized using the hematoxylin and eosin (H&E) stain. Which of the following is a silver stain used to ide...
- Pigments
- Exogenous Pigments
- Endogenous Pigments
- Which of the following is a yellow-brown, endogenous pigment found in increased amounts as cells age in organs such as nerves, heart, and liver?
- Which of the following pigments is not classified as endogenous?
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Histotechnologists, histotechnicians, pathologists' assistants, laboratory supervisors, and laboratory managers. This course is also appropriate for HTL and HT students, cytologists, and pathology residents.
Author Information: Brooke Eguia, BS, MS, HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
The author has no conflict of interest to disclose.
Reviewer Information: Kristen Meyer, HTL(ASCP)CMQIHCCM is the manager of the anatomic pathology services at Kaiser Permanente Northwest Region.
