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- Tissue Identification
- Connective Tissue
- Connective Tissue: Type I Collagen
- Connective Tissue: Type III Collagen
- Hepatocytes: Liver Epithelial Cells
- Kupffer Cells and other Sinusoidal Cells
- Type III collagen or reticular connective tissue provides architectural framework found in the form of a honeycomb network within the liver.
- Normal Histology
- Lobular Organization: Portal Tracts and Central Veins
- Portal Tracts: Bile Ducts
- Portal Tracts: Arterioles
- Portal Tracts: Portal Vein
- Parenchyma: The "Lobule"
- Parenchyma: Sinusoids
- The portal vein provides a large percentage of the blood supply to the liver; the remaining blood is supplied to the liver via the hepatic artery.
- The epithelial cells of the liver are grouped together to form functional units referred to as liver lobules.
- Technical Preparation
- Rapid Tissue Processing: Transplant Patients
- Biopsy Cycle Tissue Processing
- Cassetting: Sponge versus Wrapping
- Liver biopsies are delicate and subject to grossing artifacts. Using sponges to secure biopsy tissue in a tissue cassette has been proven to cause com...
- Section Thickness
- Step Sections
- To aid in diagnosis, it is recommended that liver biopsy samples are sectioned at what micrometer (μm) thickness?
- Prussian Blue
- Oil Red O
- Immunohistochemistry (IHC) and Immunoperoxidase (IP)
- Masson's trichrome stain is used to demonstrate connective tissue in liver biopsies, staining collagen blue.
- Reticular connective tissue cannot be visualized using the hematoxylin and eosin (H&E) stain. Which of the following is a silver stain used to ide...
- Exogenous Pigments
- Endogenous Pigments
- Which of the following is a yellow-brown, endogenous pigment found in increased amounts as cells age in organs such as nerves, heart, and liver?
- Which of the following pigments are classified as endogenous? (Choose all that apply.)
Level of instruction: Basic to intermediate
Intended audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
Author information: Brooke Eguia, BS, MS, HT(ASCP), HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Her senior year, she co-authored for aquatic toxicology research that Aquaculture published in January 2009. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College, as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP) is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.