Course Outline
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- Introduction
- Tissue Identification
- Connective Tissue
- Connective Tissue: Type I Collagen
- Connective Tissue: Type III Collagen
- Hepatocytes: Liver Epithelial Cells
- Kupffer Cells and Other Sinusoidal Cells
- True or False: Type III collagen, or reticular connective tissue, provides an architectural framework found in the form of a honeycomb network within ...
- Normal Histology
- Lobular Organization: Portal Tracts and Central Veins
- Portal Tracts: Bile Ducts
- Portal Tracts: Arterioles
- Portal Tracts: Portal Vein
- Parenchyma: The "Lobule"
- Parenchyma: Sinusoids
- True or False: The portal vein provides a large percentage of the blood supply to the liver; the remaining blood is supplied to the liver via the hepa...
- True or False: The epithelial cells of the liver are grouped together to form functional units referred to as liver lobules.
- Technical Preparation
- Fixative
- Rapid Tissue Processing: Transplant Patients
- Biopsy Cycle Tissue Processing
- Cassetting: Sponge versus Wrapping
- True or False: Liver biopsies are delicate and subject to grossing artifacts. Using sponges to secure biopsy tissue in a tissue cassette has been prov...
- Microtomy
- Artifacts
- Section Thickness
- Step Sections
- To aid in diagnosis, it is recommended that liver biopsy samples are sectioned at what micrometer (μm) thickness?
- Disease
- Stains
- Trichrome
- Reticulin
- Rhodanine
- Prussian Blue
- Oil Red O
- Immunohistochemistry and Immunoperoxidase
- True or False: Masson's trichrome stain is used to demonstrate connective tissue in liver biopsies, staining collagen blue.
- Reticular connective tissue cannot be visualized using the hematoxylin and eosin (H&E) stain. Which of the following is a silver stain used to ide...
- Pigments
- Exogenous Pigments
- Endogenous Pigments
- Which of the following is a yellow-brown, endogenous pigment found in increased amounts as cells age in organs such as nerves, heart, and liver?
- Which of the following pigments is not classified as endogenous?
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and medical laboratory science students, pathology residents, and practicing pathologists.
Author Information: Brooke Eguia, BS, MS, HT(ASCP), HTL(ASCP) is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
Reviewer Information: Kristen Meyer, HTL(ASCP)CM, QIHCCM is the manager of the anatomic pathology services at Kaiser Permanente Northwest Region.
