Course Outline
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- Calibration Overview
- What is Calibration?
- What is Calibration?
- What is a Calibrator?
- Based on the assay response recorded by the red arrow for an unknown sample in the picture, provide the best estimate for the concentration that would...
- Calibrator Safety and Handling
- Why is Calibrator Analysis Important?
- When Are Calibrators Analyzed?
- How Many Calibrators Must Be Analyzed?
- When is Calibration Not Required?
- When would recalibration most likely be necessary?
- Calibrator Set Points
- Wait! How are Calibrators "Calibrated"?
- Evaluation of Calibrations and Troubleshooting Calibration Issues
- Calibration Evaluation
- Automated vs. Manual Calibration Evaluation
- Do Calibrations of Clinical Analytes Always Produce a "Linear" Response?
- What is the most concerning aspect regarding the calibration response curve in the image?
- Calibration Verification and Linearity
- What is Calibration Verification?
- What is Linearity Verification?
- What is Analytical Measurement Range (AMR) Verification?
- True or False: A calibration curve with a low calibrator of 0.5 ng/mL and a high calibrator of 6500 ng/mL is used to quantitate an unknown patient sam...
- Troubleshooting Failures Which May Require Recalibration
- Identifying Assay Failures Using Plotted QC
- Potential Assay Drift
- Potential Assay Shift
- Complete Calibrator Swaps
- Incorrect Reagent Swaps
- Which scenario best aligns with what is observed in the QC trending data in the image?
- True or False: Both levels of QC on an analyzer have been running low for the past few analyses. New QC material from a different lot was analyzed and...
- Strategies for Mitigating Calibration Failures
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Medical laboratory supervisors, scientists, and technicians. This course is also appropriate for medical laboratory science students and pathology residents.
Author Information: Alec Saitman, PhD, DABCC (CC, TC) completed fellowship training in Clinical Chemistry and Toxicology in the Department of Pathology at the University of California, San Diego. Dr. Saitman is board certified by the American Board of Clinical Chemistry (ABCC) in Clinical Chemistry and Toxicological Chemistry. He is currently the Technical Director of the Core Laboratory at Providence Regional Laboratories in Portland, Oregon. His research focuses on the development of small molecule quantitation assays using mass spectrometry. He is particularly interested in establishing efficient production workflows in toxicology testing to meet the growing testing demands in his laboratory.
Reviewer Information: Kevin Foley, PhD, MT, DABCC is the director of Clinical Pathology for Kaiser Permanente Northwest. He has been in this position since 2009. After becoming a medical technologist, Kevin went on to earn a PhD in pharmacology. He then completed a fellowship in clinical chemistry. Kevin has also held full-time faculty positions at the University of Vermont, Northern Michigan University, and Mayo Clinic, Rochester.
