Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain (Online CE Course)

(based on 12 customer ratings)

Author: Cynthia Sampias, JD, CT(ASCP)HTL
Reviewer: Carla Shoffeitt, MSM, HT(ASCP)

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This course is intended to examine common challenges with H&E staining and offer guidance for troubleshooting.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 9/30/2023
Approved through 9/1/2022

Objectives

  • Review the basics of H&E staining and what optimal staining should look like.
  • Identify common problems encountered with staining techniques.
  • Consider solutions to common artifacts that influence stain results.
  • Discuss the advantages of staining platforms and the use of kits to standardize stain performance.
  • Address outside factors that can influence H&E stain success.
  • Identify strategies used for successful H&E staining.

Customer Ratings

(based on 12 customer ratings)

Course Outline

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Additional Information

Level of Instruction: Basic to intermediate

Intended Audience: Clinical laboratory histotechnologists, technicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.

Author information: Cynthia Sampias, JD, CT(ASCP)HTL is currently a Senior Field Support Specialist with Leica Biosystems. Her primary focus is to assist histology teams on all core histology products/consumables and troubleshooting. During her career, she worked as a Histotechnologist and Global Project Manager for Covance Central Laboratory Services and as a Senior Cytotechnologist/Histotechnologist at Diagnostic Cytology Laboratories, both located in Indianapolis, Indiana. She holds a Doctor of Jurisprudence degree from Indiana University School of Law with a specialty in health care law, and a Masters of Public Affairs degree in Health Services Administration from Indiana University Northwest. She is certified as both a cytotechnologist and histotechnologist.

Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP) is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.

Course description: This course is intended to examine common challenges with H&E staining and offer guidance for troubleshooting.

Need multiple seats for your university or lab? Get a quote



Automated H&E Stainer


Derm-lilght


Sebaceous gland showing pyknotic nuclei, visible on top half of the image. Shutterstock


drying and cracking


Good diff in eosin


Automated staining instrument with interface capability


Good staining - kidney