Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain (Online CE Course)

(based on 160 customer ratings)

Author: Cynthia Sampias, JD, CT(ASCP)HTL
Reviewer: Michael D. Glenn, HT

This course is intended to examine common challenges with H&E staining and offer guidance for troubleshooting.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 12/31/2021
Approved through 9/1/2022


  • Review the basics of H&E staining and what optimal staining should look like.
  • Identify common problems encountered with staining techniques.
  • Consider solutions to common artifacts that influence stain results.
  • Discuss the advantages of staining platforms and the use of kits to standardize stain performance.
  • Address outside factors that can influence H&E stain success.
  • Identify strategies used for successful H&E staining.

Customer Ratings

(based on 160 customer ratings)

Course Outline

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Additional Information

Level of Instruction: Basic to intermediate

Intended Audience: Clinical laboratory histotechnologists, technicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.

Author information: Cynthia Sampias, JD, CT(ASCP)HTL is currently a Senior Field Support Specialist with Leica Biosystems. Her primary focus is to assist histology teams on all core histology products/consumables and troubleshooting. During her career, she worked as a Histotechnologist and Global Project Manager for Covance Central Laboratory Services and as a Senior Cytotechnologist/Histotechnologist at Diagnostic Cytology Laboratories, both located in Indianapolis, Indiana. She holds a Doctor of Jurisprudence degree from Indiana University School of Law with a specialty in health care law, and a Masters of Public Affairs degree in Health Services Administration from Indiana University Northwest. She is certified as both a cytotechnologist and histotechnologist.

Reviewer information: Michael D. Glenn, HT is currently a Field Support Specialist at Leica Biosystems. He covers a twelve-state area within the United States, in which Michael supports, educates, and collaborates with doctors and histology professionals pertaining to the histology field. Michael received his BS from Indiana State University and Certificate in Histology from Indiana University School of Medicine. His professional background consists of research, clinical, and field support. Michael is a member of the National Society for Histotechnology.

Course description: This course is intended to examine common challenges with H&E staining and offer guidance for troubleshooting.

Automated H&E Stainer


Sebaceous gland showing pyknotic nuclei, visible on top half of the image. Shutterstock

drying and cracking

Good diff in eosin

Automated staining instrument with interface capability

Good staining - kidney