An additional technique for the detection of DNA methylation is found with the methylated DNA immunoprecipitation microarray (MeDIP-chip) for genome-wide analysis.
MeDIP-chip Steps:
- The genomic DNA is sonicated into small fragments and then immunoprecipitated with an antibody directed against 5-methylcytidine.
- Input DNA and methylated DNA can be differentially labeled with Cy5 (red) and Cy3 (green) and co-hybridized as a two-color experiment on microarrays, or used for data validation.
Note: The immunoprecipitation (IP) fraction can become the input sample to one of two DNA detection methods: array hybridization using microarrays or high-throughput sequencing.18
