Alternative Methods for Identification: DNA Sequencing

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Alternative Methods for Identification: DNA Sequencing

DNA Sequencing
  • Identifies organisms by determining the precise order of nucleotides in the DNA.
  • The sequence is then compared to a database of known sequences that are characterized by specific organisms.
  • Commonly used genes for sequence identifications are 16S RNA, rpoB, and heat shot protein 65.
  • Even with commercially available methods, sequencing is a complex and often cost-prohibitive method that requires a high level of expertise.
32. Gathany, James. "This image depicted three Enteric Diseases Laboratory Branch (EDLB) public health scientists, setting up a bacterial DNA sequencing run on a sequencing instrument." CDC Public Health Image Library (PHIL). Public domain. 2019. Accessed July 28, 2023. https://phil.cdc.gov/Details.aspx?pid=23146

DNA sequencing instrument (32)