References

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The page below is a sample from the LabCE course Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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References

American College of Rheumatology, Antinuclear Antibodies (ANA); December 2021. Available at: https://rheumatology.org/patients/antinuclear-antibodies-ana. Accessed April 19, 2023.
ANA. Lab Tests Online web site. Available at: http://labtestsonline.org/understanding/analytes/ana/tab/test/. Accessed April 19, 2023.
ANA test. Mayo Clinic web site. Available at: http://www.mayoclinic.org/tests-procedures/ana-test/basics/definition/prc-20014566. Accessed April 19, 2023.
Anuradah V, Chopra A, Sturgess A, Edmonds J. Cost-effective screening method for antinuclear antibody detection. Asian Pacific League of Associations for Rheumatology. 2004(7):13-18.
Arbuckle MR, McClain MT, Rubertone MV, et al. Development of autoantibodies before the clinical onset of systemic lupus erythematosus. N Engl J Med. Oct 16 2003;349(16):1526-1533.
Autoimmune diseases. Womenshealth.gov website. Available at: https://www.womenshealth.gov/a-z-topics/autoimmune-diseases. Accessed April 19, 2023.
Bossuyt X, Frans J, Hendrickx A, Godefridis G, Westhovens R, Marien G. Detection of Anti-SSA Antibodies by Indirect Immunofluorescence. Clin Chem. 10 7 2004;50(12):2361-2369.
Castro C, Gourley M. Diagnostic Testing and Interpretation of Tests for Autoimmunity. J Allergy Clin Immunol. Feb 2010; (2 Suppl 2): S238-S247. Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832720/. Accessed April 19, 2023.
Chan EK, Damoiseaux J, Carballo OG, Conrad K, de Melo Cruvinel W, Francescantonio PL, Fritzler MJ, Garcia-De La Torre I, Herold M, Mimori T, Satoh M, von Mühlen CA, Andrade LE. Report of the first international consensus on standardized nomenclature of antinuclear antibody HEp-2 cell patterns 2014-2015. Front Immunol. Aug. 20, 2015; 6:412. Available at: http://journal.frontiersin.org/article/10.3389/fimmu.2015.00412/full. Accessed April 19, 2023.
Clinical and Laboratory Standards Institute (CLSI). Quality Assurance of Laboratory Tests for Autoantibodies to Nuclear Antigens: (1) Indirect Fluorescence Assay for Microscopy and (2) Microtiter Enzyme Immunoassay Methods; Approved Guideline. Second ed. CLSI document I/LA02-A2. Wayne, PA: CLSI; 2006.
Delves PJ. Autoimmune Disorders. Merck Manual Consumer Version website. Available at: https://www.merckmanuals.com/home/immune-disorders/allergic-reactions-and-other-hypersensitivity disorders/autoimmune-disorders. Accessed April 19, 2023.
Dermatomyositis. Mayo Clinic website. Available at: http://www.mayoclinic.org/diseases-conditions/dermatomyositis/basics/definition/con-20020727. Accessed April 19, 2023.
Dugdale DC. Autoimmune Disorders. MedlinePlus web site. Available at: https://www.nlm.nih.gov/medlineplus/ency/article/000816.htm. Accessed April 19, 2023.
Extractable Nuclear Antigen Antibodies (ENA) Panel. Lab Tests Online website. Available at: https://www.testing.com/tests/extractable-nuclear-antigen-antibodies-ena-panel/. Accessed April 19, 2023.
Firestein GS, Budd RC, Gabriel SE, McInnes IB, O'Dell JR. Kelley's Textbook of Rheumatology. 9th ed. St. Louis: MO: W. B. Saunders: 2013.
Fritzler MJ, Hanson C, Miller J, Eystathioy T. Specificity of autoantibodies to SS-A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate. J Clin Lab Anal. 2002;16:103-108.
Fritzler MJ, Miller BJ. Detection of autoantibodies to SS-A/Ro by indirect immunofluorescence using a transfected and overexpressed human 60 kD Ro autoantigen in HEp-2 cells. J Clin Lab Anal. 1995;9:218-224.
Fritzler MJ, Wall W, Gohill J, Kinsella TD, Humbel RL. The Detection of Autoantibodies on HEp-2 Cells Using an Indirect Immunoperoxidase Kit (Colorzyme®). Diag Immunol. 1986;4:217-221.
Ilza S. Laboratory diagnosis of autoimmune diseases – new technologies, old dilemmas. Biochemia Medica. 2010;20(1):45-56. Available at: http://www.biochemia-medica.com/content/ilza-salamunic-laboratory-diagnosis-autoimmune-diseases-new-technologies-old-dilemmas. Accessed April 19, 2023.
Immunofluorescent Assays (IFA). Immuno Concepts website. Available at: https://immunoconcepts.com/products/ifa/. Accessed April 19, 2023.
Inflammation and Autoimmune Testing. Lab Tests Online website. Available at: https://www.testing.com/inflammation-and-autoimmune-testing/. Accessed April 19, 2023.
Keech CL, Howarth S, Coates T, Rischmueller M, McCluskey J, Gordon TP. Rapid and sensitive detection of anti-Ro (SS-A) antibodies by indirect immunofluorescence of 60kDa Ro HEp-2 transfectants. Pathology. 1996;28:54-57.
Keech CL, McCluskey J, Gordon TP. Transfection and overexpression of the human 60-kDa Ro/SS-A autoantigen in HEp-2 cells. Clin Immunol Immunopathol.1994;73:146-151.
Koopman W, et al (eds.). Clinical Primer of Rheumatology. Philadelphia: Lippincott Williams & Wilkins, 2003.
Kroshinsky D, Stone JH, Bloch DB, Sepehr A. Case records of the Massachusetts General Hospital. Case 5-2009. A 47-year-old woman with a rash and numbness and pain in the legs. N Engl J Med. Feb 12 2009;360(7):711-720.
McCarty GA, Valencia DW, Fritzler MJ. Antinuclear Antibodies-Contemporary Techniques and Clinical Application to Connective Tissue Disease. New York, NY: Oxford University Press, Inc: 1984.
Murray DL, Homburger HA, Horvat RT, Snyder MR, College of American Pathologists; S-C 2009: Antinuclear Antibody Screening Methods; CAP Surveys S-C Diagnostic Immunology;2009.
Parham, P. The Immune System, 4th ed. New York, NY: Garland Science 2015.
Polymyositis. Mayo Clinic website. Available at http://www.mayoclinic.org/diseases-conditions/polymyositis/basics/definition/con-20020710. Accessed April 19, 2023.
Prevalence of Autoimmune Diseases. Johns Hopkins Medicine website. Available at: https://pathology.jhu.edu/autoimmune/prevalence. Accessed April 19, 2023.
Rheumatoid Arthritis. Lab Tests Online website. Available at: https://labtestsonline.org/understanding/conditions/rheumatoid/. Accessed April 19, 2023.
Shiel WC. Antinuclear Antibody Test (ANA) Medicine.Net web site. Available at: http://www.medicinenet.com/antinuclear_antibody/article.htm#what_are_antinuclear_antibodies. Accessed April 19, 2023.
The Autoantibody Standardization Committee website. Available at: http://asc.dental.ufl.edu/. Accessed April 19, 2023.
The International Consensus on ANA Patterns (ICAP) website. Available at: www.anapatterns.org. Accessed April 19, 2023.
van Beers, J.J.B.C., Hahn, M., Fraune, J. et al.Performance analysis of automated evaluation of antinuclear antibody indirect immunofluorescent tests in a routine setting. Autoimmunity Highlights 9, 8 (2018). https://doi.org/10.1007/s13317-018-0108-y. Accessed April 19, 2023.
Wallace DJ. New methods for antinuclear antibody testing: does it cut costs and corners without jeopardizing clinical reliability? Nat Clin Pract Rheumatol. Aug 2006;2(8):410-411.
Willcocks LC, Carr EJ, Niederer HA, et al. A defunctioning polymorphism in FCGR2B is associated with protection against malaria but susceptibility to systemic lupus erythematosus. Proc Natl Acad Sci USA. Apr 27 2010;107(17):7881-7885.