Table 7 below delineates some of the more commonly seen false-negative reactions with Rh typing reagents and provides insight into the causes and corrective actions that may be undertaken when these problems are presented.
Table 7. False-Negative Reactions with Rh Typing Reagents. Likely Cause | Corrective Action |
Failure to follow manufacturer's instructions precisely | Review direction circular; repeat test |
Resuspension too vigorous | Resuspend all tubes gently during repeat testing |
Immunoglobulin-coated cells (in vivo) | Use saline-active typing reagent; repeat test |
Incorrect reagent selected | Read vial carefully; repeat test |
Reagent deterioration | Open new vial, perform appropriate QC; repeat test |