Paracoccidioides brasiliensis produces large, round yeast cells (10–50 µm) with circumferential budding, appearing as a mariner's wheel in tissue sections or primary wet preparations. The daughter cells bud on a narrow base from the mother cell.
The top right image shows a lactophenol cotton blue preparation of P. brasiliensis demonstrating budding yeast cells. The bottom right photo shows a methenamine silver stain of P. brasiliensis demonstrating the classic "mariner's wheel"—buds projecting from the yeast cell.
51. CDC/Georg. (1963). Image #4296. Under a magnification of 970X, this photomicrograph depicts budding cells of the fungus organism Paracoccidioides brasiliensis during its yeast phase. PHIL public domain. https://phil.cdc.gov/Details.aspx?pid=4296 52. CDC/Georg. (1963). Image #527. This photomicrograph of a methenamine silver-stained tissue sample revealed some of the histopathology exhibited in a case of paracoccidioidomycosis. Note the multiple buds projecting from one of the Paracoccidioides brasiliensis yeast form cells. PHIL public domain. https://phil.cdc.gov/Details.aspx?pid=527
