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The page below is a sample from the LabCE course Semen Analysis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Beginning the Analysis

Within 30-60 minutes from time of collection:

  • Assess liquefaction and appearance of the semen sample.
  • Measure semen volume.
  • Measure semen pH (if this is required).
  • Prepare a wet preparation and assess sperm motility. Also determine the dilution that will be needed to perform an accurate sperm count.
  • Assess sperm viability if the percentage of motile spermatozoa is low. If this testing is not performed in your laboratory, a comment should be included on the report that the decreased motility may be the result of either non-viable or non-motile sperm.
  • Make semen smears for assessing morphology.
  • Prepare dilutions for sperm counts and perform the counts.