Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Fundamentals of Molecular Diagnostics (retired 5/21/2020). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Fundamentals of Molecular Diagnostics (retired 5/21/2020) (online CE course) »
How to Subscribe
Individual course$20 Add to cart


Hybridization is the pairing or annealing of two strands of DNA. Hybridization is therefore based on the formation of double stranded hybrids from single stranded nucleic acids. These double stranded hybrids form under precise conditions and are detected using probes.

A probe is a set of nucleic acids of known identity which seeks out the target of interest. Depending on the detection technique, probes and/or targets can either be labeled or unlabeled and the reaction can take place with one attached to a matrix or in solution, thus dividing the techniques into two broad categories:

  • Solid phase
  • Solution phase