Blastocystis Diagnosis

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 184 CE courses, most popular
$109Add to cart
Pick Your Courses
Up to 8 CE hours
$55Add to cart
Individual course$25Add to cart
The page below is a sample from the LabCE course Intestinal Protozoan Parasites. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Intestinal Protozoan Parasites (online CE course)
Blastocystis Diagnosis

Among routine microscopic methods, the permanent stained smear is generally the best choice, although they can be seen on wet mounts they are difficult to identify. As mentioned previously, several forms or stages exist. The most commonly described "body form" or "vacuolar form" is generally round, varies from 6-40 μm, and has a large central body that looks like a vacuole.
The antigenic detection method for stool that is currently in use is the ELISA. ELISA and fluorescent antibody techniques have also been developed to detect serum antibodies.
33. Lim Zhining, Valentia. "Image shows four common forms of Blastocystis hominis - vacuolar, granular, amoeboid, and cyst forms." Wikimedia Commons, 14 May 2006, https://commons.wikimedia.org/wiki/File:Four_common_forms_of_Blastocystis_hominis_Valzn.jpg
34. DPDx. "Parasites - Blastocystis spp. - Figure A: Blastocystis sp. stained with trichrome. The nuclei in the peripheral cytoplasmic rim are visible, staining purple." CDC.gov, 21 Oct 2019, https://www.cdc.gov/dpdx/blastocystis/index.html

Wet mounts of four common forms of Blastocystis hominis (33).
Trichrome smear of a B. hominis cyst (34).