Differentiating Giardia and Dientamoeba Cysts from those of the Commensal Flagellates

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The page below is a sample from the LabCE course Intestinal Protozoan Parasites. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Differentiating Giardia and Dientamoeba Cysts from those of the Commensal Flagellates

Table 7 outlines some key features in differentiating the cysts of the flagellates.
Table 7. Differentiating Giardia and Dientamoeba Cysts from those of the Commensal Flagellates.
OrganismImageNuclei #Other Features
Giardia duodenalis
Usually oval (can be round)
Usual size: 11–14 x 7–10 μm

(27)
4 Deep-staining median bodies lie across longitudinal fibers
Shrinkage gives a halo effect (arrow)
Dientamoeba fragilis
Oval to round
5–8 μm
Cysts exist but are very rarely seen and difficult to identify. 2 Can show shrinkage
Chilomastix mesnili
Lemon or pear-shaped with hyaline knob
Usually 7–9 x 4–6 μm

(28)
1 Cytostome with supporting fibrils
Pentatrichomonas hominis No cysts
27. DPDx. (2017). Parasites: Giardia. CDC.gov.https://www.cdc.gov/dpdx/giardiasis/index.html
28. DPDx. (2019). Parasites-Chilomastix mesnili - Figure E: Cyst of C. mesnili in a stool specimen, stained with trichrome. Image taken at 1000x magnification." CDC.gov.https://www.cdc.gov/dpdx/chilomastix/index.html