Concentration Techniques

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The page below is a sample from the LabCE course Intestinal Protozoan Parasites. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Concentration Techniques

Although concentration methods are mostly considered useful for helminth eggs, they may also increase the likelihood of finding certain protozoan cysts and sporozoan oocysts. Different methods utilizing sedimentation or flotation do exist. Stool specimens can either be fresh or preserved in formalin. The zinc sulfate flotation method is more useful for certain helminth eggs; a sedimentation technique is preferred for recovery of cysts and oocysts.
The formalin-ethyl acetate sedimentation concentration technique is commonly used. Protocols for this technique can be found here.