Hepatitis B Surface Antigen - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course Immunohistochemistry (IHC) - Detection and Identification of Infectious Disease Processes in Surgical Pathology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Hepatitis B Surface Antigen

Hepatitis B Surface Antigen (HBsAg) is found in antigen positive hepatocytes in the liver of patients with type B viral hepatitis, cirrhosis, and hepatocellular carcinoma.

Mouse IgG1 monoclonal antibody clone 3E7 localizes in the cytoplasm in type B viral hepatitis infected hepatocytes. No heat-induced epitope retrieval (HIER) or proteolytic-induced epitope retrieval (PIER) pretreatment is required.

Both images demonstrate HBsAg positive liver showing cytoplasmic staining of infected hepatocytes. Mouse monoclonal antibody, clone 3E7, HRP labeled detection system, and DAB chromogen are used.