Additional Testing Methods for HER2 - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course HER2 Testing in Breast Cancer. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Additional Testing Methods for HER2

Oncotype DX^® - Genomic Health (Redwood City, California)

Reverse transcription polymerase chain reaction (RT-PCR) based assay analyzes the expression of a panel of 21 genes, among them HER2.

HERmark^® Breast Cancer Assay - Monogram Biosciences (San Francisco, California)

This assay provides direct quantitative measurements of HER2 total protein and HER2 homodimer levels in formalin-fixed paraffin-embedded (FFPE) tissue.

HER2/neu Enzyme-linked immunosorbent assay (ELISA) - Siemens Healthcare Diagnostics (Erlangen, Germany)

FDA approved "sandwich" enzyme immunoassay using mouse monoclonal antibody for capture and a different biotinylated mouse monoclonal antibody for the detection of human HER2 protein.