In simple terms, a monoclonal antibody is derived from a single cell line made by using one type of immune cell, which is a clone of a unique parent cell. It is produced by injecting a specific antigen into a mammal (mouse) to create a monoclonal antibody. In most cases, they are produced in mice, but other species have been used.
Monoclonal antibodies are more pure and have a higher affinity and selectivity than polyclonal antibodies. Monoclonal antibodies are homogeneous and are free of non-specific antibodies. These are some advantages of using this type of antibody.