Monoclonal Antibodies

The page below is a sample from the LabCE course Immunohistochemistry (IHC) Basics in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Immunohistochemistry (IHC) Basics in Histology (online CE course)
Monoclonal Antibodies

In simple terms, a monoclonal antibody is derived from a single cell line made by using one type of immune cell, which is a clone of a unique parent cell. It is produced by injecting a specific antigen into a mammal (mouse) to create a monoclonal antibody. In most cases, they are produced in mice, but other species have been used.
Monoclonal antibodies are more pure and have a higher affinity and selectivity than polyclonal antibodies. Monoclonal antibodies are homogeneous and are free of non-specific antibodies. These are some advantages of using this type of antibody.