When to Use BSL-3 Practices in a BSL-2 Laboratory

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When to Use BSL-3 Practices in a BSL-2 Laboratory

There may be times when the Laboratory Director or Safety Officer determines that enhanced precautions or ‘BSL-3 practices’ (e.g., use of double gloves, solid front gown, or respiratory protection) are needed based on a risk assessment, such as:
  • When working with organisms that may be transmitted by the inhalation route and are normally handled at BSL-3, but a BSL-3 laboratory is not readily available.
  • When certain higher-risk organisms are suspected, such as Brucella spp., Coccidioides spp., Blastomyces dermatitidis, Francisella tularensis, Histoplasma capsulatum, Mycobacterium tuberculosis, MERS-CoV, SARS, highly pathogenic influenza, etc.