Motility:
Motility by wet preparation is not recommended. Motility medium is more reliable, but shouldn't be read until 18–24 hours.
- Bacillus anthracis is non-motile (NOTE: Bacillus cereus biovar anthracis has variable motility.)
- Bacillus cereus is motile
Catalase:
Bacillus species are catalase positive. Catalase testing must be performed in a biosafety cabinet (BSC) due to the creation of aerosols.
India Ink:
India ink can reveal the capsule, but testing is not recommended.
NOTE: Bacillus cereus biovar anthracis is similar and must also be ruled out.