Using automated systems, including mass spectrometry technology, may result in exposure to dangerous pathogens. Always consider steps in the protocol that may generate aerosols. Perform as many preparatory steps as possible under the BSC, and always follow manufacturer directions for organisms.
REMEMBER: Select agents may be considered dangerous and transmissible via aerosol. Sentinel laboratories should refer the suspect isolate(s) to their LRN Reference Laboratory rather than try to identify them using an automated system.
If using mass spectrometry (MALDI-TOF) for bacterial identification, remember:
The standardized tube extraction method has been found to inactivate organisms effectively, but not in the case of Bacillus anthracis endospores. It is best NOT to perform mass spectrometry on suspect organisms, but LRN Reference laboratories should be aware of the following:
- Check with the specific manufacturer to confirm that the matrix solution provided will inactivate any suspect organisms. (Sometimes, 70% formic acid is added.)
- If the manufacturer provides your laboratory with an alternate tube extraction method, the LRN Clinical Laboratory Protocols recommend filtering the resulting extract using a 0.1–0.2 µm (or less) filter. This additional step is intended to reduce the risk of laboratory contamination with viable bacteria and spores.
