General H&E Staining Procedures

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General H&E Staining Procedures

Steps for Regressive H&E Staining:
  1. Hydrate slides
  2. Overstain in hematoxylin
  3. Tap water rinse
  4. Differentiate
  5. Tap water rinse
  6. Blue
  7. Running tap water rinse
  8. Eosin counterstain
  9. Dehydrate
  10. Clear
  11. Coverslip
Steps for Progressive H&E Staining:
  1. Hydrate slides
  2. Stain in hematoxylin
  3. Tap water wash for 15 minutes (helps to blue nuclei)
  4. Proceed with steps 8–11