A cold agglutinin titer can be used to determine the strength of the cold agglutinin and help determine clinical significance. The titers are performed at 4ºC, RT, 37ºC, and AHG. Titrations are done from 1:1 through 1:2048 dilutions using the patient's plasma and saline. Donor RBCs are then added and the combination is incubated for 2 hours and observed for agglutination.
Titers at 1:512 or greater at 4ºC are considered clinically significant. Normal individuals have a titer of less than 1:64.
9. Datta, Suvro and Berentsen, Sigbjørn. Management of autoimmune haemolytic anaemia in low-to-middle income countries: current challenges and the way forward. The Lancet Regional Health - Southeast Asia, Dec 2023. 23. 100343. 10.1016/j.lansea.2023.100343.
