Eosinophils vs. Neutrophils

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The page below is a sample from the LabCE course Body Fluid Differential Tutorial. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Eosinophils vs. Neutrophils

Eosinophils have larger, more 3-dimensional granules than those seen in neutrophils. In peripheral blood smears, eosinophils are normally bi-lobed. In cytospins this does not necessarily hold true and should not be used solely to differentiate an eosinophil from a neutrophil.
In a well-stained smear, the orange color of eosinophil granules is easily observed, which easily distinguishes eosinophils from neutrophils. However, when the slide is not stained appropriately, observing the granule size will aid in the identification of the granulocyte. An eosinophil is indicated by the arrow in the image on the right.