Acid Extends Hematoxylin Life: Why do I Need it for Differentiation?

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Acid Extends Hematoxylin Life: Why do I Need it for Differentiation?

A mild acid solution is important for clearing the nuclei and reducing overall background staining on the slide. When this step is inadequate, nuclei appear overstained and a purple haze will be noted grossly on the slide. Microscopically, mucin will be stained purple and may obscure details necessary for diagnosis.

The hematoxylin stain in this section of colon appears to be taking over
the section. Even the mucin in the goblet cells are stained blue.