Ease of Use and Reproducibility

Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain (online CE course)
Ease of Use and Reproducibility

The staining protocol for H&E follows a basic protocol:
  • Dewaxing
  • Hydration
  • Hematoxylin
  • Differentiation
  • Bluing
  • Eosin
  • Dehydration
  • Clearing
  • Coverslipping
The format is easily reproduced and the reagents are resilient enough to allow for large numbers of slides to be stained consistently before reagents need to be changed.

Good staining - placenta