Purpose of Eosin

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Purpose of Eosin

Eosin is the most commonly used counterstain to distinguish between the cytoplasm and nuclei of cells. Effective eosin staining consists of three shades with the darkest shade existing in red blood cells and eosinophils; medium shade in muscle and cytoplasm of eosinophils; and the lightest shade in connective tissue.

Another example of a well stained slide. Note the color variation of the eosin stained components.