HLA antibody binding is usually measured in terms of mean fluorescent intensity (MFI). This provides a semi-direct/quantitative means to assess the strength or affinity of HLA antibodies binding to the respective HLA antigen on the latex bead in the assay.
- The higher the MFI, the higher likelihood of that HLA antibody causing a rejection if transplantation occurs.
- Transplant programs should establish their own MFI cutoffs and initiate plasmapheresis or other therapies to lower antibody levels in the patient vascular system as needed.
