Antibody Screening Purpose and Interpretations

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The page below is a sample from the LabCE course The Human Leukocyte Antigen (HLA) System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Antibody Screening Purpose and Interpretations

HLA antibody binding is usually measured in terms of mean fluorescent intensity (MFI). This provides an semi-direct/quantitative means to assess the strength or affinity of HLA antibodies binding to the respective HLA antigen on the latex bead in the assay.

    • The higher the MFI, the higher likelihood of that HLA antibody causing a rejection if transplantation occurs.
    • Transplant programs should establish their own MFI cutoffs and initiate plasmaphoresis or other therapies to lower antibody levels in patient vascular system as needed.