Fluorescence

Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course Breast Cancer Predictive Markers. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Breast Cancer Predictive Markers (online CE course)
Fluorescence

Fluorochromes (fluorescent chromogens) are the molecules which are attached to the DNA probe that provide a method for visualization. Fluorophores on the DNA probe will fluoresce (give off) a specific color of light with exposure to certain wavelengths of light, which can be viewed only with a fluorescent microscope. When the light hits these molecules, it excites them and causes them to emit a complementary, longer wavelength of light at a lower energy level. The number and pattern of fluorescent signals observed can be used for a quantitative diagnostic evaluation of the breast tumor.