Variables Affecting IHC

Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course Breast Cancer Predictive Markers. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Breast Cancer Predictive Markers (online CE course)
Variables Affecting IHC

All factors related to each testing phase of specimen processing in the pathology laboratory may affect IHC staining.
Critical variables that affect staining results include:
  • Temperature
  • pH
  • Ionic conditions
  • Titer of antibody concentration
  • Number of epitope sites per cell
  • Duration of incubation
  • Fixation and tissue processing