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The page below is a sample from the LabCE course PCR Fundamentals: Focus on Multiplex PCR Assay and the Advantages over Singleplex Assays. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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References

Applied Biosystems. (2018). Factors influencing multiplex real-time PCR. http://www3.appliedbiosystems.com/cms/groups/mcb_marketing/documents/generaldocuments/cms_076529.pdf.

Berkowitz, F., & Jerris, R. (2016). Practical medical microbiology for clinicians. John Wiley and Sons.

Burd, E. (2010). Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev. 23:550-576.

Center for Medicare & Medicaid Services. (2018). https://www.cms.gov/regulations-and-guidance/legislation/clia/downloads/ldt-and-clia_faqs.pdf.

Center for Medicare & Medicaid Services. (2018). https://www.cms.gov/Regulations-and-Guidance/Legislation/CLIA/Downloads/6064bk.pdf.

Clinical and Laboratory Standards Institute (CLSI). (2018). Validation and verification of multiplex nucleic acid assays; Approved guideline. (2nd ed.). CLSI.

Edward, M., & Gibbs, R. (1994). Multiplex PCR: Advantages, development, and applications. Genome Res. 3:S65-S75.

Elnifero, E., Ashshi, A., Cooper, R., & Klapper, P. (2000). Multiplex PCR: Optimization and application in diagnostic virology. Clin Microbiol Rev.13:559-570.

Laboratory for environmental pathogens research. (2004). Polymerase Chain Reaction. http://www.eeescience.utoledo.edu/Faculty/Sigler/Von_Sigler/LEPR_Protocols_files/PCR.pdf.

Markoulatus, P., Siafakas, N., & Moncay, M. (2002). Multiplex polymerase chain reaction: A practical approach. J Clin Lab Anal. 16:47-51.

Mullis, K. (1990). The unusual origin of the polymerase chain reaction. Scientific American. 262: 56-61.

OIE validation guidelines 2014: Development and optimisation of nucleic acid detection assays. http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/current/GUIDELINE_3.6.3_NAD_ASSAYS.pdf.

Palavacino, E. (2015). One sample, multiple results: The use of multiplex PCR for diagnosis of infectious syndromes. Clinical Laboratory News. https://myadlm.org/cln/articles/2015/april/one-sample-multiple-results .

PCR Primer Design Guidelines. (2018). http://premierbiosoft.com/tech_notes/PCR_Primer_Design.html.

Perkel, J. (2012). Overcoming the Challenges of Multiplex PCR. Biocompare. http://www.biocompare.com/Editorial-Articles/117895-Multiplex-PCR/.

Rogers, B., Shankar, P., Jerris, R., Kotzbauer, D., & Anderson, E., (2015). Impact of a rapid respiratory panel test on patient outcomes. Arch Pathol Lab Med. 139(5):636-641. http://www.ncbi.nlm.nih.gov/pubmed/25152311.