Table 2 compares the Digene and the two Cervista HPV Tests. All three methods use signal amplification.
Table 2. Comparison of Digene and Cervista HPV DNA Tests.
| Digene High-Risk HPV HC2 DNA | Cervista HPV HR Screen | Cervista HPV 16/18 |
| Probes | - RNA cocktail of RNA strands
- Complementary to HPV-assay types
| - Cocktail of nucleotide probes; complementary to assay HPV type's target region
- Cocktail of nucleotides; complementary to one-half of target regions
| - Cocktail of nucleotide probes; complementary to type 16 and type 18 target region
- Cocktail of nucleotides; complementary to one-half of type 16 and type 18 target region
|
| Hybridization Result | Creation of DNA: RNA hybrids | Creation of 5' flaps | Creation of 5' flaps |
| Signal Detected | Chemiluminescence | Fluorescence | Fluorescence |
| Amplification Result | 3000 Fold | 1–10 Million Fold | 1–10 Million Fold |
| Test Result | Positive or negative for the presence of the assay's HR-HPV types | Positive or negative for the presence of the assay's HR-HPV types | Positive or negative for HPV 16;
positive or negative for HPV 18 |
