Holzer's Crystal Violet Staining - Chemistry - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course Histology Special Stains: Nervous Tissue. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Holzer's Crystal Violet Staining - Chemistry

Holzer's crystal violet stain is used to identify glial cells and processes. Glial cells stained with crystal violet are resistant to decolorization due to the alkalinity of the aniline-chloroform mixture.

Glial cells stained with Holzer's crystal violet. Image courtesy of Wikimedia Commons.