Sample type required: Deparaffinized and rehydrated tissue section (6–8μm) on positively (+) charged slides
Preferred fixative: 10% neutral buffered formalin (NBF)
Control: Normal spinal cord
| Reagent | Time | Technical Notes |
| 0.5% cresyl echt violet | 2 minutes | |
| Distilled water | 3 changes | |
| 70% alcohol | Differentiate until the background is colorless | Check microscopically for endpoint |
Post-staining procedure: The tissue section should be dehydrated through graded alcohols, beginning with 95% immediately following the differentiation step. Two changes of xylene should follow, followed by a coverslip.
Expected Results:
- Nissl substance - Dark blue to purple
