Synthetic dyes/stains are generally created so that they are one of the following stain types:
Basic - contains basic groups that have an affinity for acidic tissue elements.Acidic
- contains acidic groups that have an affinity for basic tissue elements.
Basic stains are used to stain nuclei and other basophilic
(base-loving) cellular structures in tissues. Acidic stains are used to stain cytoplasm and other acidophilic
(acid-loving) cellular structures in tissues. Many biological staining procedures rely on acid-base chemistry.