Table 4 is a fatty/brain processing protocol with a closed-system processor using standard processing reagents and pressure/vacuum. The tissue has been mostly fixed before processing. Notice how much more time fatty and brain tissue require in each reagent. Typically, fatty tissue and brain tissue require approximately 4 or 5 hours more processing time than routine surgicals, primarily due to the increased water content of these tissue types. Fatty tissue processed on a routine processing protocol may need reprocessing if the tissue is mushy or greasy. Brain tissue processed on a routine processing protocol may not section properly and have sections that explode in the waterbath.
Table 4. Fatty/Brain Tissue Processing with Closed-System Processor.Reagent | Time (minutes) |
10% neutral buffered formalin (NBF) | 90 |
10% NBF | 90 |
70% alcohol | 60 |
95% alcohol | 90 |
95% alcohol | 90 |
100% alcohol | 100 |
100% alcohol | 100 |
100% alcohol | 120 |
Xylene | 100 |
Xylene | 120 |
Paraffin | 90 |
Paraffin | 90 |
Paraffin | 90 |
Paraffin | 90 |