In the early 1900s, tissue processing was accomplished manually. Today, various automated tissue processors have become the “pièce de résistance” in the histology laboratory. Automated tissue processors are necessary to allow histotechnologists to focus on microtomy and staining. Although tissue processors have been in the histology laboratory for decades, today’s automated models pride themselves on being faster, safer, and more consistent than their predecessors.
After selecting the appropriate automated tissue processor, a combination of factors must be considered when deciding on the subsequent processing protocols. These protocols can directly affect the quality of processed tissue specimens. Some of the main operational factors include:
- Staffing
- Turn-around time
- Cost
- Safety
- Quality and consistency
- Tissue type and size
- Staining techniques
- Reagent availability
- Reagent recycling
- Hazardous waste disposal
Histology laboratory personnel spend excessive time optimizing processing protocols and addressing tissue processing concerns. Since a wide range of variables influences tissue processing, processing is rarely perfect, and troubleshooting skills are necessary to pinpoint the problematic step.
