Table 3: Review of the Basic Steps for Routine Paraffin Embedding

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Table 3: Review of the Basic Steps for Routine Paraffin Embedding

StepsDescription
1. Open a single cassetteExamine cassette lid and crevices for tissue fragments. Remove the lid from the cassette.
2. Identify specimen/Verify tissue typeExamine and identify the tissue type. Note the number of pieces, cut surfaces, ink, and special instructions. Check the legibility of the accession number and verify the tissue type.
3. OrientSelect the appropriate size base mold and position the specimen with the correct orientation for tissue type. Fill the mold with molten paraffin.
4. Complete blockReplace the cassette bottom and fill it with additional molten paraffin.
5. HardenAllow the block to completely cool and harden on the cold plate before attempting to remove it from the base mold.
6. ScrapeNeatly scrape excess paraffin from the edges of the block to ensure a secure fit into the block holder of the microtome.
7. InspectInspect for gross defects, such as air bubbles.