Cytogenetics

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 148 CE courses, most popular
$95Add to cart
Pick Your Courses
Up to 8 CE hours
$50Add to cart
Individual course$20Add to cart
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course Chronic Myeloid Leukemia (CML). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Chronic Myeloid Leukemia (CML) (online CE course)
Cytogenetics

Cytogenetics:
  • CML cases have the characteristic t(9;22) translocation called the Philadelphia chromosome which can be detected by karyotyping.
  • CML cases have the gene mutation BCR-ABL1 that can be detected by PCR (polymerase chain reaction) or NGS (next generation sequencing) which is a DNA based technology thats test for gene mutation.
  • The Philadelphia chromosome (BCR-ABL) can also be detected by fluorescent in situ hybridization (FISH) which is a dual color (orange & green) staining that can be examined and detected with a fluorescent microscope (fusion product appears yellow). Image A and B show CML's bcr/abl fusion; Image N is of normal unaffected cells. Images from https://www.ncbi.nlm.nih.gov/pubmed/12764379