Antigen Phenotyping

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The page below is a sample from the LabCE course The Disappearing Antibody: A Case Study. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about The Disappearing Antibody: A Case Study (online CE course)
Antigen Phenotyping

A standard follow-up to antibody identification is antigen phenotyping.
Phenotype the following:
  • Patient's red cells (expecting them to lack the corresponding antigen)
  • Donor red cells (in this case, those transfused before an antibody was identified, or, more typically, to find suitable antigen-negative donors to crossmatch prior to transfusion).
If you had wanted to type the patient for any antigens at this point in the investigation (2-weeks post-transfusion), which specimen would you have used?
Consider possible antigen typing problems and how to overcome them before proceeding to the next page.