DAT Change of Status - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course The Disappearing Antibody: A Case Study. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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DAT Change of Status

Notice that the patient's DAT is now negative (the IAT autocontrol in the panel done two weeks post-transfusion is negative).

Cell	Rh-Hr							Kell				Duffy		Kidd		Lewis		Lutheran		P	MNSs				Xg	Results
	Rh	D	C	c	E	e	C^w	K	k	Kp^a	Js^a	Fy^a	Fy^b	Jk^a	Jk^b	Le^a	Le^b	Lu^a	Lu^b	P	M	N	S	s	Xg^a	Gel IAT
1	rr	0	0	+	0	+	0	0	+	0	0	+	0	+	0	+	0	0	+	+	0	+	+	+	0	1+
2	rr	0	0	+	0	+	0	0	+	0	0	+	0	+	+	+	0	0	+	+	0	+	+	+	+	w+
3	rr	0	0	+	0	+	0	0	+	0	0	+	+	0	+	0	+	0	+	+	0	+	+	0	0	0
4	r''r	0	0	+	+	+	0	0	+	0	0	+	+	0	+	+	0	0	+	+	0	+	0	+	0	0
5	R₂R₂	+	0	+	+	0	0	+	0	0	0	+	+	+	+	0	+	0	+	+	+	0	+	0	0	w+
6	R₂R₂	+	0	+	+	0	0	+	+	0	0	+	+	+	+	0	+	0	+	+	+	0	+	0	+	w+
7	R₁R₁	+	+	0	0	+	0	0	+	0	0	0	+	0	+	0	+	+	+	+	+	0	+	0	0	0
8	R₁R₁	+	+	0	0	+	0	0	+	0	0	0	+	+	0	+	0	0	+	+	+	0	0	+	0	1+
9	R_zR₁	+	+	-	+	+	0	+	+	0	0	+	0	0	+	+	0	0	+	+	+	0	0	+	+	0
10	r'r	0	+	+	0	+	0	0	+	0	0	+	0	0	+	0	+	0	+	+	+	0	+	0	0	0
11	Auto																									0

The DAT is now negative because most or all of the transfused donor RBCs have likely been removed from circulation by the patient's anti-Jk^a.

Red cells are sensitized with IgG antibodies. Thus, anti-Jk^a usually causes extravascular hemolysis (EVH). However, because anti-Jk^a binds complement, some intravascular hemolysis (IVH) may occur if the spleen is overwhelmed by IgG-sensitized red cells and red cells are left in circulation long enough to hemolyze due to C9 binding.