A summary of the possible serological findings of a WAIHA investigation following elution is presented in Table 3 below.
Table 3. Potential Serological Findings following Elution in WAIHA.Scenario
| DAT Result (IgG and/or Complement)
| Serum/Plasma Antibody Screen/Identification
| Adsorption
|
Warm autoantibody reactive in eluate only
| Positive | Negative
| Not indicated
|
Warm autoantibody reactive in serum/plasma and eluate; no underlying alloantibodies
| Positive
| Reactive with all panel cells tested
| No reactivity after adsorption
|
Warm autoantibody reactive in serum/plasma and eluate; underlying alloantibodies that must be identified
| Positive
| Reactive with all panel cells tested
| Reactivity after adsorption
|
