When a screening test such as the rosette test indicates a significant FMH, a quantitative test is required to determine the dose of RhIG required. Tests include:
- Kleihauer-Betke acid elution test (KB test)
- Flow cytometry
- Other techniques that are less commonly used, e.g., fluorescence microscopy and enzyme-linked antiglobulin test
The KB and flow cytometry methods are briefly reviewed on the following pages.
