Specific reagents and techniques/methods of testing are used in the investigation and resolution of an ABO discrepancy. Knowing the correct reagents and techniques to employ will result in a more effective and efficient resolution. Some of the reagents and techniques/methods of testing for ABO discrepancy resolution are listed below and will be discussed in more detail on the following pages.
Reagents- Anti-A1 lectin (Dolichos biflorus)
- Anti-H lectin (Ulex europaeus)
- A2 reagent red cells
- O reagent red cells
Techniques/methods- Change incubation temperature
- Change incubation time
- Change testing method
When beginning an investigation into an ABO discrepancy, ensure that reagents are not expired and that reagents and equipment are working properly. Quality control testing is essential. Additionally, laboratory professionals should make sure that the patient sample has been properly collected and labeled according to blood bank protocols.
