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The page below is a sample from the LabCE course Normal Peripheral Blood Cells. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Segmented Neutrophils

Segmented neutrophils are also referred to as segs, polymorphonuclear leukocytes, polys, and PMNs. Segmented neutrophils are the most mature neutrophilic granulocytes present in circulating blood. The diameter of a segmented neutrophil is approximately 9-15 microns and the N:C ratio is approximately 1:3.

The cytoplasm of a segmented neutrophil resembles the cytoplasm of a band. It stains faintly pink and contains numerous fine specific granules, which stain pinkish-lilac with Wright or Giemsa stain.
A segmented neutrophil has the following characteristics:
  • The nucleus is a deep purple color, and the chromatin has a coarse, clumped texture.
  • The nucleus has from 2-5 lobes, with an average of 3.
  • The lobes are connected to each other by a fine filament or strand of nuclear membrane.
    • A filament is a thread-like strip, which is so narrow that there is no visible nuclear material between the two sides.