Dimorphic fungi - Paracoccidioides brasiliensis

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The page below is a sample from the LabCE course Fungal Infections in Humans. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Dimorphic fungi - Paracoccidioides brasiliensis

Paracoccidioides brasiliensis produces large, round yeast cells (10-50 µm) with circumferential budding, giving the appearance of a mariner's wheel in tissue sections or primary wet preparations. The daughter cells bud on a narrow base from the mother cell.
The top right image shows a lactophenol cotton blue preparation of P. brasiliensis demonstrating budding yeast cells. The bottom right image shows a methenamine silver stain of P. brasiliensis demonstrating the classic mariner's wheel budding.
Images courtesy of the CDC.