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The page below is a sample from the LabCE course Vitamin D. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Antibody and Other Interferences

For assays that use antibodies, the possibility exists for interference by heterophile antibodies (human anti -sheep, mouse, rat, or goat antibodies) in the patient sample. Patients who have been regularly exposed to animals or have received immunotherapy or diagnostic procedures utilizing immunoglobulins or immunoglobulin fragments may produce antibodies that interfere with immunoassays. These antibodies may cause erroneous results. If this is the case, it is recommended that LC - MS assays be used to avoid such interferences.
Hemolyzed samples (concentrations greater than 50 mg/dL), lipemic, or severely icteric samples may cause falsely elevated results in those assays that use spectrophotometric methods for analysis. It is recommended that these samples be avoided if possible.
Other potential interferences in the patient sample could be present and may cause erroneous results in immunoassays. Some examples that have been documented in literature include rheumatoid factor, endogenous alkaline phosphatase, fibrin, and proteins capable of binding to alkaline phosphatase.