Stains and Dyes

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The page below is a sample from the LabCE course Electrophoresis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Stains and Dyes

Substance
Stain or Dye
Comments
Proteins
Ponceau S
Coomassie Brilliant Blue
Silver
Specific for Proteins
Silver is a biohazard
Lipoproteins
Sudan Black B
Oil Red O		-
Enzymes
Enzyme substrate and a
chromagen or fluorescent dye
Reaction catalyzed by enzyme and color or fluorescence detected
Hemoglobin
Not needed

Is intensely colored
Nucleic Acids (DNA/RNA)
  1. Ethidium Bromide (EtBr)
  2. SyBr Green
  3. Silver
  1. EtBr is Carcinogenic
  2. SyBr Green is new - Introduced in 1995
  3. Silver is a biohazard