This electrophoresis gel displays the migration patterns of a person with normal hemoglobin distribution. Typically, Hb A is present more than 95%, with the remaining being Hb A2 and Hb F.
Here, you can see the large band of Hb A with a faint band in the Hb F and Hb A2 regions.
Controls for A, F, A, S, and C are included.
(AF and ASC are simply labels for the controls and do not indicate migration order.)