Normal Hemoglobin Electrophoresis

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The page below is a sample from the LabCE course Beta Thalassemia. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Normal Hemoglobin Electrophoresis

This electrophoresis gel displays the migration patterns for a person with normal hemoglobin distribution. Normally Hb A is present in excess of 95% with the remaining being made up of Hb A2 and Hb F.

Here you can see the large band of Hb A with a faint band in the Hb F and Hb A2 regions.

Controls for A and F and A, S, and C are included.
(AF and ASC are simply labels for the controls and do not indicate order of migration.)