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The page below is a sample from the LabCE course Human Papillomavirus (HPV) and Molecular Testing for Cervical Cancer. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Cobas HPV Test, continued

Roche's cobas HPV test identifies DNA from 14 high risk (HR)-HPV types commonly associated with cervical cancers, including the identification of HPV types 16 and 18, the two most common types of HPV infections (They account for approximately 70% of all cervical cancers).
The fully automated test utilizes cervical specimens that are collected in liquid-based cytology vials. The test is based on two major processes:
  1. Automated specimen preparation to simultaneously extract HPV and cellular DNA and
  2. PCR amplification of target DNA sequences using both HPV and β-globin-specific complementary primer pairs and real-time detection of cleaved fluorescent-labeled HPV and β-globin-specific oligonucleotide detection probes.
The master mix reagent for the cobas HPV Test contains primer pairs and probes specific for the 14 HR-HPV types and β-globin. Results are simultaneously reported as positive or negative for the pooled 12 HPV types (HPV31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66, and -68) from channel 1, with HPV16 and HPV18 genotypes read individually from channels 2 and 3. A fourth channel detects the human β-globin gene as a control for sample adequacy and assay inhibition.