Fat Droplets and RBCs

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The page below is a sample from the LabCE course The Urine Microscopic: Microscopic Analysis of Urine Sediment. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Fat Droplets and RBCs

Fat droplets can be mistaken for RBCs. Sudan III will selectively stain the neutral fats in bright red-orange, as indicated by the arrows in the top image.

Cholesterol can be identified by Maltese cross formation under polarized light, as seen in the bottom image.

Stained Fat Droplets.
Oval Fat Bodies Demonstrating Maltese Cross under Polarizing Light.