Suspect an autoantibody if:
- All panel and screen cells are positive and the autocontrol (AC) is positive.
- Reaction strength in all the cells is usually the same.
Cold autoantibody: The strongest reactions occur at the immediate spin phase. Reactions may weaken in strength at 37°C and AHG.
Warm autoantibody: The strongest reaction occurs at AHG. Reactions may be seen at 37°C and usually not seen at immediate spin.