When to Suspect an Autoantibody

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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When to Suspect an Autoantibody

Suspect an autoantibody if:
  • All panel and screen cells are positive and the autocontrol (AC) is positive.
  • Reaction strength in all the cells is usually the same.
Cold autoantibody: The strongest reactions occur at the immediate spin phase. Reactions may weaken in strength at 37°C and AHG.
Warm autoantibody: The strongest reaction occurs at AHG. Reactions may be seen at 37°C and usually not seen at immediate spin.